Knowledge, attitudes, and factors determining the willingness for COVID-19 vaccination among students in Bangladesh: An online-based cross-sectional study.

Aim: The most effective way to avoid COVID-19 is through immunization against the virus that causes the disease. The primary objective of this study was to assess the extent of knowledge, attitudes, acceptability, and factors influencing COVID-19 vaccination acceptance among higher secondary and university students in Bangladesh. Subject and methods: A structured questionnaire-based online survey was conducted among 451 students residing in Khulna and Gopalganj cities from February to August, 2022. The willingness to accept the COVID-19 vaccine was compared with a few covariates using the chi-square test, and we then used binary logistic regression to identify the determinants that led Bangladeshi students to receive the COVID-19 vaccine. Results: During the study period, almost 70% of the students obtained immunizations, with 56% of men and 44% of women reporting having done so. The age range of 26-30 years had the highest percentage of students who had received vaccinations, and 83.9% of students agreed that the COVID-19 vaccine is crucial for students. The results of the binary logistic regression clearly show that gender, degree of education, and respondents' willingness, encouragement, and beliefs have a significant impact on students' eagerness to receive COVID-19 vaccination. Conclusion: The rising trend in vaccination status among Bangladeshi students is highlighted by this study. Additionally, our results eloquently demonstrate that vaccination status varies by gender, education level, willingness, encouragement, and respondent's viewpoint. The outcomes of this study are essential for health policy makers and other interested parties to successfully organize their immunization program for young adults and children at various levels.

Social assistance programs during the COVID-19 pandemic in Bangladesh: Does faith-based approach matters?

This paper aimed to examine the role of faith-based organizations (FBOs) in the distribution of social assistance (SA) during the COVID-19 pandemic in Bangladesh. The paper adopts a qualitative case-study approach to explore the best practices in the distribution of SA by five (5) organizations including FBOs, governmental organizations (GOs), Non-governmental organizations (NGOs), political groups, and private voluntary groups. In this study, 14 beneficiaries of SA were selected by stratified proportionate random sampling method, and five (5) key personnel were selected purposively based on their experiences in the management of SA distribution. The study found that "no-move, no touch" approach followed by FBOs is more likely to establish human rights and social justice and reduce the transmission of diseases. In contrast to the modern idea, which undermines the faith-based charity, the study proved that faith-based charity is gaining attraction as an effective approach combating global pandemic. The findings of this paper will be useful for policymakers, voluntary service workers, GO, and NGO workers to ensure the distribution of SA in a more productive and disciplined way during and after an emergency like the COVID-19 pandemic.

4-Aminoquinolines modulate RNA structure and function: Pharmacophore implications of a conformationally restricted polyamine.

RNA structure plays an important role in regulating cellular function and there is a significant emerging interest in targeting RNA for drug discovery. Here we report the identification of 4-aminoquinolines as modulators of RNA structure and function. Aminoquinolines have a broad range of pharmacological activities, but their specific mechanism of action is often not fully understood. Using electrophoretic mobility shift assays and enzymatic probing we identified 4-aminoquinolines that bind the stem-loop II motif (s2m) of SARS-CoV-2 RNA site-specifically and induce dimerization. Using fluorescence-based RNA binding and T-box riboswitch functional assays we identified that hydroxychloroquine binds the T-box riboswitch antiterminator RNA element and inhibits riboswitch function. Based on its structure and riboswitch dose-response activity we identified that the antagonist activity of hydroxychloroquine is consistent with it being a conformationally restricted analog of the polyamine spermidine. Given the known role that polyamines play in RNA function, the identification of an RNA binding ligand with the pharmacophore of a conformationally restricted polyamine has significant implications for further elucidation of RNA structure-function relationships and RNA-targeted drug discovery.

Prediction of COVID-19 Pandemic in Bangladesh: Dual Application of Susceptible-Infective-Recovered (SIR) and Machine Learning Approach.

The outbreak of COVID-19 is a global problem today, and, to reduce infectious cases and increase recovered cases, it is relevant to estimate the future movement and pattern of the disease. To identify the hotspot for COVID-19 in Bangladesh, we performed a cluster analysis based on the hierarchical k-means approach. A well-known epidemiological model named "susceptible-infectious-recovered (SIR)" and an additive regression model named "Facebook PROPHET Procedure" were used to predict the future direction of COVID-19 using data from IEDCR. Here we compare the results of the optimized SIR model and a well-known machine learning algorithm (PROPHET algorithm) for the forecasting trend of the COVID-19 pandemic. The result of the cluster analysis demonstrates that Dhaka city is now a hotspot for the COVID-19 pandemic. The basic reproduction ratio value was 2.1, which indicates that the infection rate would be greater than the recovery rate. In terms of the SIR model, the result showed that the virus might be slightly under control only after August 2022. Furthermore, the PROPHET algorithm observed an altered result from SIR, implying that all confirmed, death, and recovered cases in Bangladesh are increasing on a daily basis. As a result, it appears that the PROPHET algorithm is appropriate for pandemic data with a growing trend. Based on the findings, the study recommended that the pandemic is not under control and ensured that if Bangladesh continues the current pattern of infectious rate, the spread of the pandemic in Bangladesh next year will increase.

RNA sequence and ligand binding alter conformational profile of SARS-CoV-2 stem loop II motif.

Antiviral drug discovery continues to be an essential complement to vaccine development for overcoming the global pandemic caused by SARS-CoV-2. The genomic RNA of SARS-CoV-2 contains structural elements important for viral replication and/or pathogenesis making them potential therapeutic targets. Here we report on the stem-loop II motif, a highly conserved noncoding RNA element. Based on our homology model we determined that the G to U transversion in the SARS-CoV-2 stem-loop II motif (S2MG35U) forms a C-U base-pair isosteric to the C-G base-pair in the early 2000's SARS-CoV (S2M). In addition, chemo-enzymatic probing and molecular dynamics simulations indicate the S2MG35U conformational profile is altered compared to S2M in the apical loop region. We explored S2MG35U as a potential drug target by docking a library of FDA approved drugs. Enzymatic probing of the best docking ligands (aminoglycosides and polymyxins) indicated that polymyxin binding alters the conformational profile and/or secondary structure of the RNA. The SARS-CoV-2 stem-loop II motif conformational differences due to nucleotide transversion and ligand binding are highly significant and provide insight for future drug discovery efforts since the conformation of noncoding RNA elements affects their function.

Psychosocial stress and trauma during the COVID-19 pandemic: Evidence from Bangladesh.

The COVID-19 outbreak has become a global health crisis affecting both the physical and mental health of people across the world. Likewise, the people of Bangladesh are going through a menacing mental health catastrophe with the outbreak of coronavirus that resulting in stress and trauma. Hence, this situation is altering people's lifestyles and generating complexities in psychological well-being. The study was based on the review of published articles and media reports related to stress and trauma during the COVID-19 pandemic in Bangladesh. A total of 10 peer-reviewed articles and 45 newspaper reports were included following an extensive literature search. The contents were searched on Google, Google Scholar, PubMed, local online newspapers, social networking sites, and different webpages and published articles in different journals on COVID-19 from March 5 to October 25, 2020. The review study finds that the mental health of people in Bangladesh has severely been affected by the outbreak of coronavirus. All of the government, voluntary, and civil organizations need to give further emphasis on psychosocial and bereavement counseling in order to support those experiencing mental shocks resulting from the COVID-19 crisis. There is the need to strengthen more consultative and collaborative efforts from all public health experts, social workers, psychologists, and policymakers in doing so. Social workers in this context will be able to make meaningful contributions in supporting those affected people to better adjust to the challenging situation.